Identification of Avian Toll-Like Receptor 3 and 7 and Analysis of Gene Variation Sites

Toll-like receptors 3 and 7 (TLR3 7) mediate immune responses through the recognition of viral single-stranded RNA double-stranded therefore play important roles in host defense. Differences TLR3 or may affect resistance to infection. To illuminate these differences, partial coding sequence (CDS) genes were cloned amplified from Phasianus colchicus Numida meleagris, total 64 avian species sequences later analyzed. Based on results, 315 non-synonymous mutation sites 202 synonymous also observed TLR3, 227 174 TLR7. Among sites, 44 45 functional regions 7, used common variation amino acids most species. A number different appeared visualized. H59Y, E60K, G64R, E93K, L112S, K117E, N118K, R120H, V123M, L163F, R443Q, R459K, E460D, C485H, F511L for I432V, M437V, T732S TLR7 considered. It is possible that bind ligands crucial recognition. These data indicated positive selection has occurred genes.